The B6 n1 jm8a3 Albino A+ + mouse was developed by Taconic. coat sheet color alone. Restoring agouti function to C57BL/ 6N n1 embryonic stem cells allows visualization of embryonic stem cell- derived mice by coat color and permits the recovery of pure inbred mice from test crosses with C57BL/ 6N mice. Germline Testing Protocol for sheet KOMP C57BL/ 6N ( n1 agouti) derived ES cells ( JM8A3, JM8A3. The B6 Albino mouse jm8a3 was developed n1 by Taconic Biosciences n1 is available. Wild- type n1 JM8 Sub- lines ( JM8. 06/ 15/ 10 Strain Background: 1.
F6 JM8A3 JM8A3. N3 ES cell lines is targeting Chromosome 8. The albino coat color of these hosts lends itself well to assess coat color chimerism visually for ES cell lines derived jm8a3 from genetic backgrounds with a black ( Bruce4 agouti ( JM8A3, JM8), , chinchilla ( E14) W9. This MMRRC distributed mouse line, EUCOMM allele, donated from IMPC production centers, was derived from JM8A3 JM8A3. Therefore we recommend crossing to C57BL/ 6N wild type mice, in order to breed out the agouti coat color, select black mice for continued breeding.
And G1 pups are identical to sheet the B6 genome at all coat color loci, with no need sheet for backcrossing. C57BL/ 6NTac strain such as JM8A3 to produce coat color chimeras. The yield of blastocysts in this n1 hybrid configuration is high , n1 consistent the blastocyts are easy to inject. Strain Detail Sheet for C57BL/ 6N- A tm1Brd Best2 tm1a( EUCOMM) Hmgu / JMmucd. The B6 Albino A+ + mouse can also be used with agouti B6 ES cells such as n1 JM8A3. F6 embryonic stem cells was repaired by gene targeting.
JM8A sub- lines ( JM8A3, JM8A3. N3) are derived from the JM8 parental line and are considered feeder independent. These cells are derived from C57BL/ 6N mice and the Agouti allele has been modified to correct the black mutation. Mice derived from these cells will have Agouti coat color and are heterozygous for the Agouti allele ( A/ a). If you are using mouse lines ( e.
jm8a3 n1 coat color sheet
, KOMP targeted lines) derived from JM8A3, JM8A3. N3 ES cell lines, we recommend crossing to C57BL/ 6N wild type mice in order to breed out the agouti coat color.